Journal: Frontiers in Microbiology

Article Title: Rapid Detection of mecA and femA Genes by Loop-Mediated Isothermal Amplification in a Microfluidic System for Discrimination of Different Staphylococcal Species and Prediction of Methicillin Resistance

doi: 10.3389/fmicb.2020.01487

Figure Lengend Snippet: The sequences of loop-mediated isothermal amplification (LAMP) primers.

Article Snippet: Reference strains involved in this study to evaluate the specificities of the LAMP assays were as follows: S. aureus (CMCC 26003), S. epidermidis (ATCC 49134), S. haemolyticus (CGMCC 1.10528), S. hominis (ATCC 27844), Staphylococcus warneri (ATCC 17917), Staphylococcus capitis (ATCC 35661), Staphylococcus cohnii (ATCC 29974), S. aureus (ATCC 43300), Staphylococcus argenteus (CGMCC 1.802), Staphylococcus lugdunensis (ATCC 700328), Staphylococcus saprophyticus (ATCC 49907), Staphylococcus auricularis (ATCC 33753), Staphylococcus chromogenes (ATCC 43764), Staphylococcus pettenkoferi (DSM 19554), Staphylococcus pseudintermedius (ATCC 51874), Escherichia coli (CMCC 44102), Klebsiella pneumoniae (CMCC 46117), Pseudomonas aeruginosa (CMCC 10211), Acinetobacter baumannii (CICC 22933), Enterococcus faecium (ATCC 35667), Enterobacter cloacae (CMCC 43501), Stenotrophomonas maltophilia (CICC 22935), Streptococcus pneumoniae (CMCC 31001), Acinetobacter calcoaceticus (ATCC 23055), Proteus mirabilis (ATCC 7002), and Streptococcus pyogenes (ATCC 19615).

Techniques: Amplification

Journal: Frontiers in Microbiology

Article Title: Rapid Detection of mecA and femA Genes by Loop-Mediated Isothermal Amplification in a Microfluidic System for Discrimination of Different Staphylococcal Species and Prediction of Methicillin Resistance

doi: 10.3389/fmicb.2020.01487

Figure Lengend Snippet: Results of the LOD tests. (A1–E1) Amplification curves of the LOD tests for S. aureus , S. epidermidis , S. haemolyticus , S. hominis , and methicillin-resistant S. aureus , respectively, and (A2–E2) the corresponding melting curve analysis. LOD, limit of detection; dF/dT, derivative of the fluorescence/derivative of the temperature; SAU, Staphylococcus aureus ; SEP, Staphylococcus epidermidis ; SHA, Staphylococcus haemolyticus ; SHO, Staphylococcus hominis ; MRSA, methicillin-resistant Staphylococcus aureus ; NTC, no template control.

Article Snippet: Reference strains involved in this study to evaluate the specificities of the LAMP assays were as follows: S. aureus (CMCC 26003), S. epidermidis (ATCC 49134), S. haemolyticus (CGMCC 1.10528), S. hominis (ATCC 27844), Staphylococcus warneri (ATCC 17917), Staphylococcus capitis (ATCC 35661), Staphylococcus cohnii (ATCC 29974), S. aureus (ATCC 43300), Staphylococcus argenteus (CGMCC 1.802), Staphylococcus lugdunensis (ATCC 700328), Staphylococcus saprophyticus (ATCC 49907), Staphylococcus auricularis (ATCC 33753), Staphylococcus chromogenes (ATCC 43764), Staphylococcus pettenkoferi (DSM 19554), Staphylococcus pseudintermedius (ATCC 51874), Escherichia coli (CMCC 44102), Klebsiella pneumoniae (CMCC 46117), Pseudomonas aeruginosa (CMCC 10211), Acinetobacter baumannii (CICC 22933), Enterococcus faecium (ATCC 35667), Enterobacter cloacae (CMCC 43501), Stenotrophomonas maltophilia (CICC 22935), Streptococcus pneumoniae (CMCC 31001), Acinetobacter calcoaceticus (ATCC 23055), Proteus mirabilis (ATCC 7002), and Streptococcus pyogenes (ATCC 19615).

Techniques: Amplification, Fluorescence, Control

Journal: Frontiers in Microbiology

Article Title: Rapid Detection of mecA and femA Genes by Loop-Mediated Isothermal Amplification in a Microfluidic System for Discrimination of Different Staphylococcal Species and Prediction of Methicillin Resistance

doi: 10.3389/fmicb.2020.01487

Figure Lengend Snippet: Results of the specificity tests. (A1–E1) Amplification curves of the specificity tests for S. aureus , S. epidermidis , S. haemolyticus , S. hominis , and methicillin-resistant S. aureus , respectively, and (A2–E2) the corresponding melting curve analysis. dF/dT, derivative of the fluorescence/derivative of the temperature; SAU, Staphylococcus aureus ; MSSA, methicillin-susceptible Staphylococcus aureus ; SEP, Staphylococcus epidermidis ; SHA, Staphylococcus haemolyticus ; SHO, Staphylococcus hominis ; SWA, Staphylococcus warneri ; SCA, Staphylococcus capitis ; SCO, Staphylococcus cohnii ; MRSA, methicillin-resistant Staphylococcus aureus ; SAR, Staphylococcus argenteus ; SLU, Staphylococcus lugdunensis ; SAP, Staphylococcus saprophyticus ; SAI, Staphylococcus auricularis ; SCH, Staphylococcus chromogenes ; SPE, Staphylococcus pettenkoferi ; SPS, Staphylococcus pseudintermedius ; ECO, Escherichia coli ; KPN, Klebsiella pneumoniae ; PAE, Pseudomonas aeruginosa ; ABA, Acinetobacter baumannii ; EFA, Enterococcus faecium ; ECL, Enterobacter cloacae ; SMA, Stenotrophomonas maltophilia ; SPN, Streptococcus pneumoniae ; ACA, Acinetobacter calcoaceticus ; PMI, Proteus mirabilis ; SPY, Streptococcus pyogenes ; NTC, no template control.

Article Snippet: Reference strains involved in this study to evaluate the specificities of the LAMP assays were as follows: S. aureus (CMCC 26003), S. epidermidis (ATCC 49134), S. haemolyticus (CGMCC 1.10528), S. hominis (ATCC 27844), Staphylococcus warneri (ATCC 17917), Staphylococcus capitis (ATCC 35661), Staphylococcus cohnii (ATCC 29974), S. aureus (ATCC 43300), Staphylococcus argenteus (CGMCC 1.802), Staphylococcus lugdunensis (ATCC 700328), Staphylococcus saprophyticus (ATCC 49907), Staphylococcus auricularis (ATCC 33753), Staphylococcus chromogenes (ATCC 43764), Staphylococcus pettenkoferi (DSM 19554), Staphylococcus pseudintermedius (ATCC 51874), Escherichia coli (CMCC 44102), Klebsiella pneumoniae (CMCC 46117), Pseudomonas aeruginosa (CMCC 10211), Acinetobacter baumannii (CICC 22933), Enterococcus faecium (ATCC 35667), Enterobacter cloacae (CMCC 43501), Stenotrophomonas maltophilia (CICC 22935), Streptococcus pneumoniae (CMCC 31001), Acinetobacter calcoaceticus (ATCC 23055), Proteus mirabilis (ATCC 7002), and Streptococcus pyogenes (ATCC 19615).

Techniques: Amplification, Fluorescence, Control

Journal: Frontiers in Microbiology

Article Title: Rapid Detection of mecA and femA Genes by Loop-Mediated Isothermal Amplification in a Microfluidic System for Discrimination of Different Staphylococcal Species and Prediction of Methicillin Resistance

doi: 10.3389/fmicb.2020.01487

Figure Lengend Snippet: Comparison of the results obtained by conventional methods and our system.

Article Snippet: Reference strains involved in this study to evaluate the specificities of the LAMP assays were as follows: S. aureus (CMCC 26003), S. epidermidis (ATCC 49134), S. haemolyticus (CGMCC 1.10528), S. hominis (ATCC 27844), Staphylococcus warneri (ATCC 17917), Staphylococcus capitis (ATCC 35661), Staphylococcus cohnii (ATCC 29974), S. aureus (ATCC 43300), Staphylococcus argenteus (CGMCC 1.802), Staphylococcus lugdunensis (ATCC 700328), Staphylococcus saprophyticus (ATCC 49907), Staphylococcus auricularis (ATCC 33753), Staphylococcus chromogenes (ATCC 43764), Staphylococcus pettenkoferi (DSM 19554), Staphylococcus pseudintermedius (ATCC 51874), Escherichia coli (CMCC 44102), Klebsiella pneumoniae (CMCC 46117), Pseudomonas aeruginosa (CMCC 10211), Acinetobacter baumannii (CICC 22933), Enterococcus faecium (ATCC 35667), Enterobacter cloacae (CMCC 43501), Stenotrophomonas maltophilia (CICC 22935), Streptococcus pneumoniae (CMCC 31001), Acinetobacter calcoaceticus (ATCC 23055), Proteus mirabilis (ATCC 7002), and Streptococcus pyogenes (ATCC 19615).

Techniques: Comparison

Journal:

Article Title: Novel Non- mecA -Containing Staphylococcal Chromosomal Cassette Composite Island Containing pbp4 and tagF Genes in a Commensal Staphylococcal Species: a Possible Reservoir for Antibiotic Resistance Islands in Staphylococcus aureus

doi: 10.1128/AAC.48.5.1823-1836.2004

Figure Lengend Snippet: Map depicting the regions of high nucleotide sequence similarity between the left portion of SCCpbp4 (L-C region), including both ccr genes, and the left extremities of SCCmec elements and non-mec-containing SCC elements, based on the nucleotide sequences deposited in the DDBJ/EMBL/GenBank database under accession no. AY271717 (Dallas CA-MRSA strain 2314, SCCmec type IV), AB096217 (strain MR108, SCCmec type IVc), AE016744 (S. epidermidis ATCC 12228, SCCpbp4), D86934 (strain N315, SCCmec type II), AB063172 (strain CA05, SCCmec type IVa), AP004822 (strain MW2, SCCmec type IVa), AB063173 (strain 8/6-3P, SCCmec type IVb), AB063171 (S. hominis ATCC 27844, SCC12263), AB033763 (NCTC 10442, SCCmec type I), and AB037671 (S. aureus strain 82/2082, SCCmec type III). Conserved ORFs of >99 bp are indicated by solid rectangles beneath the maps of the nucleotide sequences. Arrows above the rectangles for the map of SCCpbp4 indicate the direction of transcription. Identical colors for sequences from different elements indicate regions of >90% nucleotide sequence homology. Asterisk indicates a 28-bp sequence identity with SE0039 from SCCpbp4. Vertical arrowhead indicates the junction between the left SCC element and the chromosome.

Article Snippet: 6.5-kb element termed IE25923, present to the left of the type IVc SCC mec ( 11 ). fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG. 2. caption a7 Map depicting the regions of high nucleotide sequence similarity between the left portion of SCC pbp4 (L-C region), including both ccr genes, and the left extremities of SCC mec elements and non- mec -containing SCC elements, based on the nucleotide sequences deposited in the DDBJ/EMBL/GenBank database under accession no. {"type":"entrez-nucleotide","attrs":{"text":"AY271717","term_id":"34577204"}} AY271717 (Dallas CA-MRSA strain 2314, SCC mec type IV), {"type":"entrez-nucleotide","attrs":{"text":"AB096217","term_id":"29539351"}} AB096217 (strain MR108, SCC mec type IVc), {"type":"entrez-nucleotide","attrs":{"text":"AE016744","term_id":"27316888"}} AE016744 ( S. epidermidis ATCC 12228, SCC pbp4 ), {"type":"entrez-nucleotide","attrs":{"text":"D86934","term_id":"13785452"}} D86934 (strain N315, SCC mec type II), {"type":"entrez-nucleotide","attrs":{"text":"AB063172","term_id":"19352029"}} AB063172 (strain CA05, SCC mec type IVa), AP004822 (strain MW2, SCC mec type IVa), {"type":"entrez-nucleotide","attrs":{"text":"AB063173","term_id":"17025993"}} AB063173 (strain 8/6-3P, SCC mec type IVb), {"type":"entrez-nucleotide","attrs":{"text":"AB063171","term_id":"18148881"}} AB063171 ( S. hominis ATCC 27844, SCC 12263 ), {"type":"entrez-nucleotide","attrs":{"text":"AB033763","term_id":"7592605"}} AB033763 (NCTC 10442, SCC mec type I), and {"type":"entrez-nucleotide","attrs":{"text":"AB037671","term_id":"14020964"}} AB037671 ( S. aureus strain 82/2082, SCC mec type III).

Techniques: Sequencing